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This chapter describes how to design (combinatorial) scar-less DNA assembly protocols using the web-based software j5. j5 assists biomedical and. Here, we report the development and deployment of a web-based software tool, j5, which automates the design of scar-less multipart
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Eighty years have passed since a young Cambridge don named Herbert Butterfield published in a slender volume entitled The Whig. The former Master of Peterhouse, Herbert Butterfield, has become something of a Less a book than a lengthy essay, The