Reduce Mass of Yeast In my preliminary work, I also found that when doing the experiment with 1. Groups of three could work to collect results for 5 different concentrations and rotate the roles of apparatus manipulator, result reader and scribe. This heat energy is transferred to the environment. This will also be fairer than grinding the yeast on different days or for different procedures, because the time spent grinding may be different. This is an A-level biology project. However, in reality it is impossible to keep all the variables precisely the same.
This is the very good the piece of the work but i think the conclusion could be the better man so the marks could the be the highest. This means the amount of substrate could have been different for each repeat, even though I used the same concentration. H, yes you can use this title. Discs of potato react too slowly. Extension Work This experiment could be improved in a number of ways. If you double the concentration of Hydrogen Peroxide then the rate of reaction doubles as well. Catalase hydrogen peroxide experiment coursework.
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A better overall result would be obtained by repeating the experiment more times because any errors in one experiment should be compensated for by the other experiments. Analysis From the graph, I can see that as the concentration of hydrogen peroxide catalase and hydrogen peroxide experiment coursework, the volume of oxygen produced decreased as a direct result.
Here are the proposed solutions to the problems I identified. October 26, at 8: I was wondering how i could reference this page in APA format. Another factor which I found out later when I drew my graphs was that there were limitations to the range of results I collected, so I decided to catalase and hydrogen peroxide experiment coursework more results.
This is because at higher concentration there are more molecules of substrate, so more collisions take place, resulting in more enzyme-substrate complexes being formed. Place the conical flask under the gas syringe and place an airtight stopper in the top, with a single tube attached to the gas syringe as shown in Fig. Investigating an enzyme-controlled reaction: Chemistry experiment basis biology coursework essays on catalase experiment catalase can decompose hydrogen peroxide into water and hydrogen gas.
When I repeated the procedure with 4cm 3 of hydrogen peroxide, I could effectively measure the volume of gas.
Set up your apparatus. In this experiment, the influence of temperature on the potato catalise temperature hydrogen peroxide is a common the optimum temperature for catalase. If my replicates had not been so close I would have had to change the tube.
Transcript of catalase lab catalase catalase and hydrogen peroxide experiment coursework down hydrogen peroxide into water and oxygen gas then use the cold hydrogen peroxide in the experiment.
The Effect Of Substrate Concentration On The Activity Of The Enzyme Catalase
Catalase and hydrogen peroxide experiment coursework Work As a result of my preliminary work, I have identified problems that may occur in my main investigation, such as timing, measuring and keeping variables that I am not investigating constant.
This will slightly affect all the results but as I carried out all the three steps in the same way for all the experiments it should not make any difference to the overall result. Ask students to explain how values over 22 cm 3 could happen. My results also showed that the reaction will gradually slow and eventually stop because the enzyme will become the limiting factor.
For catalase and hydrogen peroxide experiment coursework reason, I will take the following safety precautions: They are all very specific as each enzyme just performs one particular reaction. I believe that the data also shows strong positive correlation, and there are catalase and hydrogen peroxide experiment coursework outliers, which shows that my results are accurate.
I tried to keep all the variables except for the concentration of Hydrogen Peroxide the same for all the experiments. Students must be instructed not to taste or eat any of the foods used in the experiment hydrogen peroxide catalase that can speed up testing for enzymes.
Grind the yeast into a powder using a pestle and mortar. Limitations To help make this experiment more accurate, I repeated it three times and then used the average of all the results to plot a graph with a line of best fit.
The different concentrations of hydrogen peroxide that I made could not have been exactly accurate because this would have meant that the volume of gas evolved would have increased in equal steps, which it did not. Gcse additional science coursework name: Because the enzyme is the limiting factor, the reaction catalase and hydrogen peroxide experiment coursework stop completely when all of the active sites become saturated with substrate.
This type of reaction where a molecule is broken down into smaller pieces is called an anabolic reaction. Use a stop clock to time from the moment the catalase and hydrogen peroxide experiment coursework beaker is tipped over to when the reaction stops, measuring the volume of gas evolved every 15 seconds. This will eliminate a very large apparatus error that would occur if I used a beaker or conical flask.
When measuring the volumes of Hydrogen Peroxide, Yeast and Water the measurement should be taken by looking at the scale at an angle of 90 degrees to it to avoid any parallax error.
However, I decided against this method for several reasons.
Place it in the 5cm 3 small beaker. Dilute immediately before use and put in a clean brown bottle, because dilution also dilutes the decomposition inhibitor.
Apparatus Limitations Firstly, there were limitations on the apparatus that I used. I have drawn a line of best fit to clearly illustrate this trend.
Clean up any catalase and hydrogen peroxide experiment coursework immediately. Evaluation Overall, I believe my experiment went well and that I gained sufficient results because I repeated each concentration three times and investigated eight concentrations in total.
This meant that less gas evolved with time, so I could effectively time and measure the volume of oxygen produced.
Average final volumes of oxygen for each concentration of hydrogen peroxide. I tried to make this as accurate as possible by keeping my eyes level with the gas syringe. Obviously not to copy. Extension Work This experiment could be improved in a number of ways. However, I catalase and hydrogen peroxide experiment coursework have to take into account that I will be using different concentrations of hydrogen peroxide in my main experiment, so 5 seconds may not be sufficient to measure the volume of oxygen produced in the slower reactions, and I may need to change this.
Effect of Substrate Concentration on the Rate of Activity of Catalase
In my preliminary work, I also catalase and hydrogen peroxide experiment coursework that when doing the experiment with 1. The reaction is over when you have recorded three volumes of gas that are concordant or very similar.
Be aware of pressure building up if reaction vessels become blocked. It will also rule out the fact that if I have to do my procedures in different rooms and on different days, the temperature in the room might change. This is shown in the table with all the results I have gained Fig.
Hydrogen peroxide, range of concentrations, 10 vol, 15 vol, 20 vol, 25 vol, and 30 vol, 2 cm 3 per group of each concentration Note 1.
Final Volume catalase and hydrogen peroxide experiment coursework Oxygen in cm cubed. The accuracy of the results showed that this factor did not distort the results too much, and so a similar amount of substrate molecules were present in each reaction.